In vitro Motility 1

Recombinant T. gondii MyosinA was produced in insect cells, immunoaffinity purified and adsorbed to nitrocellulose-coated coverslips. TRITC-phalloidin labeled actin filaments were added to the immobilized motor complexes, and actin filament displacement in the presence of ATP was captured by videomicroscopy. Filament displacement is shown in real time. See PMC4215259 for details.